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quantikine elisa kit  (R&D Systems)


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    R&D Systems quantikine elisa kit
    Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+quantikine+elisa+kits/Human+M-CSF+Quantikine+ELISA+Kit/pm42821460-65-17-22
    Average 94 stars, based on 68 article reviews
    quantikine elisa kit - by Bioz Stars, 2026-10
    94/100 stars

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    Control:

    Article Title: Baricitinib and Infliximab Mitigate the Endothelial-to-Mesenchymal Transition (EndMT) Induced by Cytokines in HUVECs.
    Article Snippet: Western blot images were captured using an iBright FL1500 Imaging System (Thermo Fisher Scientific) and analyzed with iBright Analysis Software (version 5.0). .. The amounts of TNFα, IL-1β, IL-6, IFNγ, and TGF-β secreted in the culture medium of the control (CM_cont) and Spike S1-activated macrophages (CM_S1) were measured with specific Human Quantikine ELISA kits (R&D Systems, Bio-Techne, Milano, Italy). .. Statistical analyses were performed using GraphPad Prism 9 (GraphPad Software, Boston, MA, USA).

    Article Title: Baricitinib and Infliximab Mitigate the Endothelial-to-Mesenchymal Transition (EndMT) Induced by Cytokines in HUVECs
    Article Snippet: Western blot images were captured using an iBright FL1500 Imaging System (Thermo Fisher Scientific) and analyzed with iBright Analysis Software (version 5.0). .. The amounts of TNFα, IL-1β, IL-6, IFNγ, and TGF-β secreted in the culture medium of the control (CM_cont) and Spike S1-activated macrophages (CM_S1) were measured with specific Human Quantikine ELISA kits (R&D Systems, Bio-Techne, Milano, Italy). .. Statistical analyses were performed using GraphPad Prism 9 (GraphPad Software, Boston, MA, USA).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Baricitinib and Infliximab Mitigate the Endothelial-to-Mesenchymal Transition (EndMT) Induced by Cytokines in HUVECs.
    Article Snippet: Western blot images were captured using an iBright FL1500 Imaging System (Thermo Fisher Scientific) and analyzed with iBright Analysis Software (version 5.0). .. The amounts of TNFα, IL-1β, IL-6, IFNγ, and TGF-β secreted in the culture medium of the control (CM_cont) and Spike S1-activated macrophages (CM_S1) were measured with specific Human Quantikine ELISA kits (R&D Systems, Bio-Techne, Milano, Italy). .. Statistical analyses were performed using GraphPad Prism 9 (GraphPad Software, Boston, MA, USA).

    Article Title: Levels of Growth Differentiation Factor 15 Correlated with Metabolic Dysfunction-Associated Steatotic Liver Disease in Children.
    Article Snippet: .. Quantification of GDF15 in plasma samples was conducted by using Human Quantikine ELISA kits (R & D Systems Inc., Minneapolis, MN, USA) according to the manufacturer’s instructions. ..

    Article Title: Cytokines from Macrophages Activated by Spike S1 of SARS-CoV-2 Cause eNOS/Arginase Imbalance in Endothelial Cells.
    Article Snippet: .. The amount of TNFα, IL-1β, IL-6, and IFNγ secreted in the culture medium of Spike S1-activated macrophages (CM_S1) was determined with the Human Quantikine ELISA kits (R&D Systems, Bio-Techne, Milano, Italy), specific for each cytokine, as specified by the manufacturer. ..

    Article Title: Extracellular vesicles derived from clonal mesenchymal stromal cells preconditioned by indirect hypoxia modulate immune responses in diabetic mice more effectively than directly preconditioned vesicles
    Article Snippet: .. To measure cytokines in both direct and indirect cMSC-CCM, the levels of IL-10, IL-6, IL-1β, TGF-β, IFN-γ, TNF-α, VEGF, and IGF-1 were measured via DuoSet ® ELISA kits (DY217B, DY206, DY201, DY240, DY285B, DY210, DY293B, DY291, R&D Systems, USA), and the amounts of HGF and bFGF were measured via human Quantikine ® ELISA kits (DHG00 and DFB50, R&D Systems, USA) according to the manufacturer’s instructions. .. For the in vivo functional assay, the concentrations of serum cytokines, including IL-4, IL-10, TNF-α, and IL-6, were measured via commercial Mouse Quantikine ELISA kits (M4000B, M1000B, MTA00B, and M6000B, respectively), following the manufacturer’s instructions (R&D Systems, Minneapolis, MN, USA).

    Article Title: Baricitinib and Infliximab Mitigate the Endothelial-to-Mesenchymal Transition (EndMT) Induced by Cytokines in HUVECs
    Article Snippet: Western blot images were captured using an iBright FL1500 Imaging System (Thermo Fisher Scientific) and analyzed with iBright Analysis Software (version 5.0). .. The amounts of TNFα, IL-1β, IL-6, IFNγ, and TGF-β secreted in the culture medium of the control (CM_cont) and Spike S1-activated macrophages (CM_S1) were measured with specific Human Quantikine ELISA kits (R&D Systems, Bio-Techne, Milano, Italy). .. Statistical analyses were performed using GraphPad Prism 9 (GraphPad Software, Boston, MA, USA).

    Article Title: Comparative efficacy of a standardized herbal composition LN22199, nicotinamide riboside, and their combination on NAD+ metabolism in healthy aging adults
    Article Snippet: The biomarker concentrations were measured using the Neurofilament light chain (NFL) ELISA kit (Cat# abx152468; Abbexa, Cambridge, UK). .. The interleukin-6 (IL-6, Cat# HS600C), tumor necrosis factor-alpha (TNF-α, Cat# HSTA00E), and growth differentiation factor 15 (GDF-15, Cat# DGD150) were quantified using Human Quantikine ELISA kits procured from R&D Systems (Minneapolis, MN). ..

    Article Title: Somatostatin Effect on Growth Factors in Hepatocellular Carcinoma
    Article Snippet: .. Methods IGF-1, HGF, VEGF and SCF were measured using the Human Quantikine ELISA kits (R&D Systems, Minneapolis, MN, USA). .. ELISA for Gastrin I was from Invitrogen (Waltham, MA, USA).

    Clinical Proteomics:

    Article Title: Levels of Growth Differentiation Factor 15 Correlated with Metabolic Dysfunction-Associated Steatotic Liver Disease in Children.
    Article Snippet: .. Quantification of GDF15 in plasma samples was conducted by using Human Quantikine ELISA kits (R & D Systems Inc., Minneapolis, MN, USA) according to the manufacturer’s instructions. ..

    Article Title: Levels of Growth Differentiation Factor 15 Correlated with Metabolic Dysfunction-Associated Steatotic Liver Disease in Children
    Article Snippet: After centrifuging the blood at 3000 rpm for 15–20 min, the plasma was stored in tubes at −80 °C and then used to perform an enzyme-linked immunosorbent assay (ELISA). .. Quantification of GDF15 in plasma samples was conducted by using Human Quantikine ELISA kits (R & D Systems Inc., Minneapolis, MN, USA) according to the manufacturer’s instructions. ..



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    Disruption of the NEDD4-AQP5 axis impairs barrier function and promotes cytokine release. (A) MUC5AC amounts (pg/mL) in cellular culture supernatants were assayed with <t>ELISA</t> across various treatment groups. (B) TER (Ω·cm²) of HNEpC monolayers was measured utilizing a Millicell ERS-2 system across the specified groups. (C, D) The amounts of IL-6 (pg/mL) <t>and</t> <t>IL-8</t> (pg/mL) in cellular culture supernatants were determined via ELISA. Data are shown as mean ± SD from three independent experiments, with each sample measured in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001 vs. Ctrl group; # P < 0.05, ## P < 0.01, ### P < 0.001 compared with the HDM group. Ctrl, untreated control; HDM, house dust mite-sensitized HNEpCs; HDM + siNEDD4, HDM-sensitized HNEpCs transfected with siNEDD4; HDM + ovAQP5, HDM-sensitized HNEpCs transfected with AQP5 overexpression plasmid.
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    Disruption of the NEDD4-AQP5 axis impairs barrier function and promotes cytokine release. (A) MUC5AC amounts (pg/mL) in cellular culture supernatants were assayed with ELISA across various treatment groups. (B) TER (Ω·cm²) of HNEpC monolayers was measured utilizing a Millicell ERS-2 system across the specified groups. (C, D) The amounts of IL-6 (pg/mL) and IL-8 (pg/mL) in cellular culture supernatants were determined via ELISA. Data are shown as mean ± SD from three independent experiments, with each sample measured in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001 vs. Ctrl group; # P < 0.05, ## P < 0.01, ### P < 0.001 compared with the HDM group. Ctrl, untreated control; HDM, house dust mite-sensitized HNEpCs; HDM + siNEDD4, HDM-sensitized HNEpCs transfected with siNEDD4; HDM + ovAQP5, HDM-sensitized HNEpCs transfected with AQP5 overexpression plasmid.

    Journal: Frontiers in Immunology

    Article Title: The role of NEDD4-mediated ubiquitination of AQP5 in allergic rhinitis: implications for nasal mucosal barrier function

    doi: 10.3389/fimmu.2026.1908261

    Figure Lengend Snippet: Disruption of the NEDD4-AQP5 axis impairs barrier function and promotes cytokine release. (A) MUC5AC amounts (pg/mL) in cellular culture supernatants were assayed with ELISA across various treatment groups. (B) TER (Ω·cm²) of HNEpC monolayers was measured utilizing a Millicell ERS-2 system across the specified groups. (C, D) The amounts of IL-6 (pg/mL) and IL-8 (pg/mL) in cellular culture supernatants were determined via ELISA. Data are shown as mean ± SD from three independent experiments, with each sample measured in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001 vs. Ctrl group; # P < 0.05, ## P < 0.01, ### P < 0.001 compared with the HDM group. Ctrl, untreated control; HDM, house dust mite-sensitized HNEpCs; HDM + siNEDD4, HDM-sensitized HNEpCs transfected with siNEDD4; HDM + ovAQP5, HDM-sensitized HNEpCs transfected with AQP5 overexpression plasmid.

    Article Snippet: Cell culture supernatants were used to detect IL-6, IL-8, and MUC5AC levels in the supernatant by using ELISA kits for IL-6 (R&D Systems, D6050), IL-8 (R&D Systems, D8000C), and MUC5AC (R&D Systems, DY2197).

    Techniques: Disruption, Cell Culture, Enzyme-linked Immunosorbent Assay, Control, Transfection, Over Expression, Plasmid Preparation